Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms i. Anything in or on which we grow a microorganism is termed a medium. Web to pdf convert any web pages to highquality pdf files while retaining page layout, images, text and. To demonstrate good aseptic technique in culture transfer or inoculation and in handling. Transfer bacterial cells from one plate to another using the. Isolate single bacterial colonies by the streakplating method. Pure culture techniques university of pennsylvania. Transfer of measured volumesdrops of culturesterile solutions dry. Pdf semesterlong assessment of aseptic technique in. Isolation and characterization of methane utilizing bacteria from wetland paddy ecosystem. A sterile medium is one which is free of all life forms.
Oxygen transfer performances in intensive microbial cultures are compared with those occuring in coalescing and noncoalescing mineral media. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Aseptic techniques refer to any method used to sterilize and maintain the sterility of an object or location. Isolation and characterization of methane utilizing bacteria. Asepsis is the state of remaining free from pathogenic and contaminating microorganisms. Microbiology is the study of small life, or microbes. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Aseptic technique is a fundamental and important laboratory skill in the field of microbiology. Sterilization is the process of rendering a medium or material free of all forms of life.
An introduction to microbiology, aseptic technique and safety preparation. Pdf isolation and characterization of methane utilizing. Choose from 224 different sets of isolation techniques flashcards on quizlet. Activated sludge activated sludge is a type of secondary treatment whose primary role is to remove most of the dissolved solids remaining in the waste stream after. Get a printable copy pdf file of the complete article 126k, or click on a page image below to browse page by page. Testing sensitivity to antimicrobial substances 21. Full text full text is available as a scanned copy of the original print version. Alternatively, if too many bacteria are plated, then overlap of cells may occur and. Microbiologists and health workers use this technique to prevent. Aseptic technique and transfer of microorganisms biology. Learn isolation techniques with free interactive flashcards.
Use pdf download to do whatever you like with pdf files on the web and regain control. An introduction to microbiology, aseptic technique and safety. It reduces bacterial contamination since the bacteria, even if they get into the plate in. Conventional microbiology plating techniques such as the spread or pour plate method is used to measure bacterial growth.
Aseptic transfer of cultures and sterile solutions 19 preparing cultures for class use 19 part 2. General aseptic techniques in microbiology laboratory. Using your free hand, pick up the tube containing the mixed culture and gently. This technique ensures a contaminant free environment while handling micro organisms. An aseptic technique experiment was conducted to test the effects of aseptic technique on the transfer of target bacteria, sarcina aurantiaca s. Aseptic technique and the transfer of microorganisms. Proper aseptic technique prevents contamination of cultures from. Free microbiology book available for download openstax. In working with microorganisms we must also have a sterile nutrientcontainingmedium in which to grow the organisms. Techniques for obtaining pure cultures from a mixed population will be described in lab 3.
1268 1004 1327 1225 291 900 881 1199 31 929 1353 222 1171 548 1213 556 1389 1058 807 560 150 1086 106 1185 656 1092 1382 815 378 878 160 1397 9 572 484